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KMID : 0364820220580030150
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Korean Journal of Microbiology
2022 Volume.58 No. 3 p.150 ~ p.156
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Prevalence of multidrug-resistant (MDR) Escherichia coli in untreated effluents from a wastewater treatment plant (WWTP) in Dhaka, Bangladesh
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Hossain Md Naeem
Roy Asim kumar
Habib Humayra
Hossain Zenat Zebin
Akhter Humaira
Begum Anowara
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Abstract
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The widespread use of antibiotics in human and animal husbandry results in the potential release of antibiotics into surrounding environments and leads to the emergence of antibiotic-resistant bacteria and antibiotic resistance genes.
With this concern, we aimed to study the molecular mechanisms associated with multidrug resistance (MDR) and biofilm formation capacity in Escherichia coli isolates from Dhaka City’s wastewater treatment plant. A total of 37 antimicrobialresistant E. coli isolates were analyzed for the presence of genes associated with antibiotic resistance and biofilm formation using several phenotypic and polymerase chain reaction (PCR) assays and curing plasmids through the SDS method. Among the 37 isolates, most prevalent gene detected was qnrS (32.43%), followed by blaTEM (29.73%), blaCTX-M-15 (24.32%), dfrA17 (21.62%), sul2 (8.1%), qnrB (5.41%), sul1 (5.41%), and dfrA1 (2.70%). Twelve E. coli isolates (32.43%) possessed only class 1 integrons. A comparison of results prior to and after plasmid curing revealed most E. coli had plasmid-mediated antibiotic resistance. Among the 37 isolates, nine strains showed weak biofilm production and one showed strong biofilm production. All of the isolates were curli producers; fimH and csgA genes were present in 72.9% and 64.9% of the isolates, respectively. The dynamic antibiotic resistance diversity revealed in this study may pose concern regarding the potential development of drug-resistant bacteria and their dissemination into the surrounding environment via WWTPs.
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KEYWORD
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antibiotic-resistant bacteria (ARBs), antibiotic resistance genes (ARGs), extended spectrum β-lactamase (ESBL)
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